Imaging Core Facility & ZEISS Microscopy Survey

Question Title

* 1. What types of imaging service have you ever used in the Imaging Core Facility at GSU? (Select all that apply)

Laser Scanning Confocal Microscopy (LSM700 and/or LSM780)

Wide Field Microscopy (Bright Field, Phase Contrast, DIC, etc.)

Transmission Electron Microscopy

Scanning Electron Microscopy / Microanalysis (EDAX)

None

Never heard about the Imaging Core Facility

Other (please specify)

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* 2. For electron microscopy imaging, what spatial resolution is required for your research?

μm

N/A

Other (please specify)

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* 3. What type of samples are you using in your research? (Select all that apply)

Fixed

Live

Antibody Stained

Fluorescence Protein Expression

Cleared Sample

Organoids

Whole Organs

Sections

Cells

Other (please specify)

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* 4. What type of sample holder/vessel are you using? (Select all that apply)

Coverslip

Non- Coverslip

Glass

Plastic

Multi-Well Plates - 6 Well

Multi-Well Plates - 12 Well

Multi-Well Plates - 24 Well

Multi-Well Plates - 96 Well

Multi-Well Plates - 384 Well

Other (please specify)

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* 5. Where do you have your imaging experiment done if you can not find a suitable microscope in the Imaging Core Facility? (Select all that apply)

Facilities in other institutes such as Georgia Tech., Emory

Purchase microscope in my own lab (or pursued PI to make purchase)

Collaboration with other labs that have the capability

Move onto different project and forget about it

Other (please specify)

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* 6. How important are the following technologies to your research?

	Not Important	Important	Extremely Important	I Don't Know
Confocal	Confocal Not Important	Confocal Important	Confocal Extremely Important	Confocal I Don't Know
Multi-photon	Multi-photon Not Important	Multi-photon Important	Multi-photon Extremely Important	Multi-photon I Don't Know
Airyscan	Airyscan Not Important	Airyscan Important	Airyscan Extremely Important	Airyscan I Don't Know
GPU-Based Deconvolution	GPU-Based Deconvolution Not Important	GPU-Based Deconvolution Important	GPU-Based Deconvolution Extremely Important	GPU-Based Deconvolution I Don't Know
Structured Illumination Microscopy	Structured Illumination Microscopy Not Important	Structured Illumination Microscopy Important	Structured Illumination Microscopy Extremely Important	Structured Illumination Microscopy I Don't Know
D-Storm	D-Storm Not Important	D-Storm Important	D-Storm Extremely Important	D-Storm I Don't Know
Lattice SIM	Lattice SIM Not Important	Lattice SIM Important	Lattice SIM Extremely Important	Lattice SIM I Don't Know
Light Sheet	Light Sheet Not Important	Light Sheet Important	Light Sheet Extremely Important	Light Sheet I Don't Know
Correlative light-electron Microscopy	Correlative light-electron Microscopy Not Important	Correlative light-electron Microscopy Important	Correlative light-electron Microscopy Extremely Important	Correlative light-electron Microscopy I Don't Know
Slide Scanner	Slide Scanner Not Important	Slide Scanner Important	Slide Scanner Extremely Important	Slide Scanner I Don't Know
High-Throughput Imaging	High-Throughput Imaging Not Important	High-Throughput Imaging Important	High-Throughput Imaging Extremely Important	High-Throughput Imaging I Don't Know
DNA-PAINT	DNA-PAINT Not Important	DNA-PAINT Important	DNA-PAINT Extremely Important	DNA-PAINT I Don't Know
Fluorescence lifetime imaging microscopy	Fluorescence lifetime imaging microscopy Not Important	Fluorescence lifetime imaging microscopy Important	Fluorescence lifetime imaging microscopy Extremely Important	Fluorescence lifetime imaging microscopy I Don't Know

Please provide details on how a specific imaging technique would support your research:

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* 7. Rank the following in order of importance:

Resolution

Sensitivity

Speed

Imaging Depth

Contrast Methods (Phase Contrast, DIC, Polarization, etc.)

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* 8. What are your resolution requirements for your top three applications?

	Lateral Resolution X/Y	Z Resolution	Time Resolution
Application A	Application A Lateral Resolution X/Y menu	Application A Z Resolution menu	Application A Time Resolution menu
Application B	Application B Lateral Resolution X/Y menu	Application B Z Resolution menu	Application B Time Resolution menu
Application C	Application C Lateral Resolution X/Y menu	Application C Z Resolution menu	Application C Time Resolution menu

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* 9. What imaging modality is important for you? (Select all that apply)

Tiles/Mosaic

Position

Optical section

Time series

Section μm <10

Section μm <10-50

Section μm <50-100

Section μm >100

N/A