Cyanine fluorophore derivatives with enhanced photostability

Nat Methods. 2011 Nov 13;9(1):68-71. doi: 10.1038/nmeth.1774.

Abstract

Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzyl Alcohols / chemistry
  • CHO Cells
  • Carbocyanines / chemistry*
  • Chromans / chemistry
  • Cricetinae
  • Cyclooctanes / chemistry
  • DNA / chemistry
  • Drug Stability
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Humans
  • Kinetics
  • Molecular Dynamics Simulation
  • Photobleaching / drug effects
  • Receptors, Dopamine D2 / metabolism
  • Singlet Oxygen / analysis

Substances

  • Benzyl Alcohols
  • Carbocyanines
  • Chromans
  • Cyclooctanes
  • Fluorescent Dyes
  • Receptors, Dopamine D2
  • cyanine dye 5
  • Singlet Oxygen
  • 4-nitrobenzyl alcohol
  • DNA
  • 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid